展望
出处:按学科分类—生物科学 农业出版社《植物细胞培养手册》第170页(1621字)
不亲和杂交中,有生活力胚数随胚年龄而减少,培养发育早期胚是有益的。应加强研究主要作物逐级幼胚培养条件和要求。这项基础研究将能大大地加速作物改良,或用常规育种或遗传工程。
最近资料指出植物对人工培养的反应是受遗传控制的。这种能力似可整合任何基因型。其遗传模式类似“修饰者”基因。Jacobsen等(1978)用马铃薯花药培养结果提出经杂交和选择,可将“弱反应”诸基因型组合,以产生“高反应”的。作者认为胚培养研究新方向是鉴别不同作物的“高反应”基因型,或经杂交和选择创造新基因型,用此作为转移“人工培养可培养性”特性转移给商品栽培品种,以利于将来遗传改良。并列入种质资源库备用。
【参考文献】:
〔1〕Bhojwani,S.S.and M.K.Razdan 1983 Zygotic embryo culture.In: Plant Tissue Culture,Theory and Practice.pp.199-235,Elesevier,Amsterdam,Monnier,M.1978 Culture of zygotic embryos.In:Frontiers of plant Tissure Culture.(T.A.Thorpe ed.)pp.277-286,Univ.of Calgary Press,Calgary.
〔2〕Nostog,K.1979 Embryo Culture as a tool in the study of comparative and development morphology.In:Plant Cell and Tissue Culture.(W.R.Sharp,P.O.Larsen,E.F.Paddock and V.Roghavan eds.)pp.179-202,Ohio State Univ.Press,Columbus.
〔3〕Raghavan,V.1976 Experimental Embryogenesis in Vascular Plants.Academic Press,New York.
〔4〕Raghavan V.,1977 Applied aspects of embryo culture.In:Applied and Fundamental Aspects of Plant Cell,Tissue and Organ Culture(J.Reincrt and Y.P.S.Bajaj eds.)pp.375-397,Springer Verlag,Berlin.
〔5〕Raghavan,V.1980 Embryo culture In: Perspectives in Plant Cell and Tissu e Culture.International Review of Cytology Suppl.11B(I.K.Vasil ed.)pp.209-240 Academic Press,New York.
〔6〕Wardlaw,C.W.1965 Physiology of embryonic development in conmophytes.Encyclopedia of Plant Physiology,vol.15/1(W.Ruhland ed.)pp.844-965,Springer Verlag,Berlin.