分生组织培养和微繁殖

出处:按学科分类—生物科学 农业出版社《植物细胞培养手册》第88页(996字)

本章讨论茎分生组织、苗尖和顶芽的离体培养技术。分生组织培养的外植体无论是采用顶端圆盖(顶端分生组织),还是更多地采用带少数叶原基的顶端分生组织(亚顶端分生组织区)。苗尖培养的外植体通常选择2cm或者比2cm更短的生长枝条的嫩尖。芽培养包括顶芽、腋芽培养,通常用生长或休眠枝条的带芽茎段进行培养。

【参考文献】:

〔1〕Andesson,W.C.1980 Mass propagation by tissue culture: prineiples and practics.In: Preceedings Conference on Nursery Production of Fruit Plants Through Tissue Culture-Applications and Feasibility SEA,Agri.Res.Results,N.E.Series No.11,pp.1-1 USDA Beltsville.

〔2〕Kartha,K.K.1981 Meristem culture and cryopreservation-methods and application In: Plant Tissue Culture(T.A.Thorpe ed.)pp.181-211.

〔3〕Murashige,T.1974 Plant propagation through tissue cultures.Annu.Rev.Plant physiol.25∶135-166.

〔4〕Wang,P.J and Hu,C.Y.1980 Regeneration of virus-free plants through in vitro culture.In: Adyances in Biochemieal Enginn ering,Vol.18,Plant Cell Cultures I(A.Fiechter, edpp.61-99.Springer-verlag,Berlin,Heidelberg,New York.

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