离体胚培养

出处:按学科分类—生物科学 农业出版社《植物细胞培养手册》第158页(1514字)

Hannig(1904)开创了胚培养的开发和农业应用新领域。他用完全无菌技术培养成熟胚(萝卜属与辣根菜属)于无机盐加糖培养基上,取得可移栽幼苗。从此曾应用胚培养以开发胚发育需要的营养和物理条件;避免种子休眠、缩短育种周期;测验种子生活力;提供微克隆材料源;拯救不亲和杂交的未成熟杂种胚,这是当今应用最广的。现已有72属植物胚离体培养成功。并有已发表过10余篇综合评论。本文介绍胚培养技术和评述培养要求和其应用。

【参考文献】:

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〔2〕Nostog,K.1979 Embryo Culture as a tool in the study of comparative and development morphology.In:Plant Cell and Tissue Culture.(W.R.Sharp,P.O.Larsen,E.F.Paddock and V.Roghavan eds.)pp.179-202,Ohio State Univ.Press,Columbus.

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